H19基因的单核苷酸多态性与猪生长性状的相关分析及其时空表达

doi:10.11843/j.issn.0366-6964.2016.05.003

Abstract

Abstract:

An attempt was made to reveal the function of H19 gene in skeletal muscle development in pigs in this study.The distribution of H19 in different tissues and dynamic expression at different developmental stages in skeletal muscle were determined by Quantitative real-time PCR(qRT-PCR).The sites of single nucleotide polymorphisms (SNPs) in its exons and introns were identified by pool DNA sequencing，and individual SNPs were genotyped using mass spectrum in Large White pig population.The trait-marker association analysis was carried out between H19 genotypes and growth performance.The expression profiles showed that H19 RNA was expressed in all tissues detected，including heart，liver，spleen，lung，kidney，intestine，stomach and skeletal muscle (leg muscle and longissimus muscle)，with remarkably high expression in skeletal muscle and kidney.Meanwhile，H19 expression existed only before postnatal 40 days and reached the peak at 105 days in embryo period during the development of skeletal muscle.Correlation analysis between SNPs and traits showed the RS15 was significantly associated with corrected age of animals with body weight of 100 kg(P=0.046 2<0.05)，RS20 was associated with the corrected eye muscle area of animals with body weight of 100 kg(P=0.009 5<0.01)；The correlation analysis between haplotypes and traits shown that RS15 had a close linkage with RS16，and their haplotype was significantly correlated with corrected backfat thickness(P=0.049 8<0.05).RS17 was closely linked to RS18，and their haplotype was also significantly correlated with corrected eye muscle area of animals with body weight of 100 kg (P=0.037 1<0.05).In conclusion，H19 gene is involved in regulation of pig skeletal muscle development and is a candidate gene for meat traits.

CLC Number:

S828
S813.3

SUN Hao，WANG Zi-shuai，XU Yin-xue，TANG Zhong-lin. The Correlation between H19 Single Nucleotide Polymorphism and Pig Growth Characters and Its Spatio-temporal Expression[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 870-881.

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The Correlation between H19 Single Nucleotide Polymorphism and Pig Growth Characters and Its Spatio-temporal Expression

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